This section focuses on an overview of nisin powder as well as its manufacturing processes and uses. Also, please check out the introduction to nisin manufacturers, supply chain issues, nisin suppliers, and nisin prices. Sinofi can help you buy high-quality nisin in bulk for your industry applications at wholesale prices based on E234 and CAS 1414-45-5.

Table of Contents

What is Nisin?

Nisin is a naturally occurring antimicrobial peptide produced by certain strains of the bacterium Lactococcus lactis during fermentation. It functions as a bacteriocin, meaning it inhibits the growth of other bacteria, particularly gram-positive organisms that can cause food spoilage and foodborne illness. Nisin is a polypeptide containing 34 amino acids. It has gained significant importance in food preservation and other applications due to its effective antimicrobial properties and safety profile. According to the U.S. Food and Drug Administration (FDA), nisin is approved to be used in the food industry. Nisin has also been evaluated by major regulatory bodies like JECFA, ECHACHEM, and WHO.

CAS No.: 1414-45-5 or 977127-33-5

According to the American Chemical Society, the CAS number of nisin is 1414-45-5 or 977127-33-5.
Molecular Formula: C143H230N42O37S7
Molecular Mass: 3354.07
Appearance: White to off-white crystalline powder

E number: E234

According to the European Food Safety Authority (EFSA), nisin is approved to be used as food additive E234.

Bulk Nisin Manufacturers

The global nisin production capacity is dominated by several key manufacturers while featuring numerous emerging players. Leading nisin manufacturers like International Flavors & Fragrances (IFF) have extensive production facilities and superior quality control systems in many countries. They have strong research capabilities and a global distribution network. Continued investment in research, technology, and customer service remains crucial for maintaining a competitive advantage in this evolving market.
Sinofi is a bulk nisin manufacturer in China, and we have a manufacturing facility with 2,500 square meters for the production of nisin E234. We supply high-quality nisin powder at wholesale prices and serve food and pharmaceutical industries worldwide.

Manufacturing Process of Nisin

The manufacturing of nisin is a complex process involving careful control of fermentation conditions, sophisticated purification techniques, and stringent quality control measures.

Strain Development and Maintenance

Microorganism Selection

Lactococcus lactis subspecies

High-yielding strains
Genetic stability
Production consistency
Quality attributes
Regulatory compliance

Production Process

Pre-fermentation

Seed Culture

Strain activation
Growth conditions
Purity checking
Viability assessment
Volume scaling
Transfer timing

Scale-up Process

Sequential scaling
Growth monitoring
Quality checks
Transfer procedures
Contamination prevention
Process documentation

Main Fermentation

Critical Parameters

Temperature control
pH maintenance
Dissolved oxygen
Nutrient feeding
Foam control
Process monitoring

Downstream Processing

Centrifugation

Continuous operation
Speed optimization
Temperature control
Clarity requirements

Filtration

Membrane selection
Flow rates
Pressure control
Cleaning procedures

Chromatography

Resin selection
Operating conditions
Elution parameters
Fraction collection

Final Purification

Saturated adsorption resin is poured into the resin column to elute impurities.
Desorbent is added for desorption to obtain a desorption solution.
The pH of the desorption solution is adjusted to 2.0-3.0, and sodium chloride is added for salting out.
The salting out solution is filtered, rotary evaporated, and dried at a low temperature to obtain high-purity nisin powder.

Nisin manufacturing requires precise control of multiple parameters throughout the production process. Success depends on maintaining optimal conditions during fermentation, efficient downstream processing, and stringent quality control. Continuous process optimization and documentation are essential for consistent, high-quality production.

Bulk Nisin Suppliers

The global nisin supply is dominated by established suppliers while featuring emerging players from various regions. Top nisin suppliers like IFF and DSM are increasingly focusing on sustainable production methods and environmentally friendly packaging solutions to meet growing market demands for responsible manufacturing practices. They maintain their positions through quality, innovation, and comprehensive customer support. Traditional market leaders face increasing competition from emerging nisin suppliers, particularly from Asia. The future of the nisin supply will likely be shaped by clean-label trends, sustainability requirements, and the development of new applications beyond traditional food preservation.
For food manufacturers seeking nisin suppliers, careful evaluation of suppliers’ quality standards, technical support capabilities, and supply chain reliability remains crucial for establishing successful long-term partnerships in this critical ingredient category.
Sinofi is a bulk nisin supplier in China, and we have a warehouse with 1,200 square meters for high-quality nisin E234. We supply different grades of nisin powder at bulk prices to food and pharmaceutical manufacturers worldwide.

Supply Chain of Nisin

The global nisin supply chain represents a complex network of processes, stakeholders, and regulatory requirements that ensure the consistent delivery of this crucial food preservative to manufacturers worldwide. Success in this market depends on maintaining strong relationships with nisin suppliers, implementing robust quality systems, and adapting to changing market demands.
The nisin market is experiencing several important developments: the Clean Label Movement.
Growing consumer demand for natural preservatives has increased interest in nisin as a clean-label solution. Leading suppliers are responding with new product formulations that align with clean-label requirements.

Wholesale and Buy Nisin in Bulk

Successful wholesale procurement of nisin requires understanding market dynamics, maintaining quality standards, and implementing effective purchasing strategies. Regular market monitoring and supplier evaluation ensure continuous improvement in the procurement process. As the global nisin market continues to expand, and nisin buyers seek effective natural preservatives to meet consumer demand for clean-label products, nisin buyers must maintain flexibility while implementing robust procurement strategies to ensure cost-effective access to quality nisin products. By following these guidelines and maintaining strong supplier relationships, nisin buyers can ensure reliable access to high-quality products while optimizing their procurement costs.
As a bulk nisin manufacturer and supplier in China, we supply high-quality nisin powder to large food and pharmaceutical manufacturers worldwide. We also wholesale nisin to small customers. Normally, they want to buy nisin powder in small quantities. To serve them, we can wholesale nisin powder at bulk prices and provide a one-stop purchasing service.

Nisin Prices (USD/Kg)

Food Grade Nisin Price (FCC, Hydrous Potency >=1000 IU/mg)

Volume	Unit Price	Shipment
50g	17 USD	Express
500g	84 USD	Express
1Kg	120 USD /Kg	Express
20Kg	25% cheaper	Express
100Kg-Above	negotiable	Express / Sea

Nisin prices have seen significant fluctuations in recent years due to changing market dynamics and growing demand across various industries. The market demonstrates complex pricing dynamics influenced by multiple factors across the supply chain.
They vary significantly based on concentration levels and quality grades. Standard food-grade nisin (2.5% concentration, 1000 IU/mg) typically ranges from $80 to $150 per kilogram in the wholesale market. Higher concentrations command premium prices. These prices reflect typical market conditions but can fluctuate based on various factors.

Key Price Determinants

Production Costs

Manufacturing costs significantly impact nisin pricing, primarily driven by:

Fermentation process expenses.
Raw material costs, particularly growth media components.
Energy consumption in production facilities.
Quality control and testing procedures.
Packaging and storage requirements.

Market Demand Factors

Price variations often correlate with:

Growing demand in food preservation applications.
Expansion of clean-label product segments.
Increased adoption in emerging markets.
Seasonal fluctuations in food production.
Regional regulatory changes.

Supply Chain Influences

Supply chain dynamics affecting prices include:

Transportation and logistics costs.
Storage and handling requirements.
Import/export regulations.
Currency exchange rates.
Global trade conditions.

Furthermore, the above price quotations of nisin, especially for small orders, do not include the express shipping fee. The reason is that the shipment cost of nisin may exceed the value of the sample. But the bulk wholesale nisin prices will be much lower than those of small orders. Please contact us for more details.

Nisin Uses

Nisin is a polycyclic antibacterial peptide that has been safely used as a food preservative for over 50 years. Its natural origin and proven safety record have made it a preferred choice in many applications, particularly as consumers increasingly demand natural food additives.

Food Industry Applications

Cheese Production

Prevent late blowing caused by Clostridium bacteria.
Extend the shelf life of processed cheese.
Control spoilage in cheese spreads.
Inhibit pathogenic bacteria growth.

Other Dairy Applications

Preserve pasteurized milk.
Extend the shelf life of yogurt.
Protect ice cream from bacterial contamination.
Maintains freshness in dairy-based beverages.

Fresh Meat Preservation

Control bacterial growth in packaged meats.
Extend refrigerated shelf life.
Reduce spoilage in vacuum-packed products.
Enhance food safety.

Processed Meat Applications

Preserve cured meats
Protect against Listeria in deli meats
Maintain quality in ready-to-eat meat products
Control bacterial growth in sausages

Canned Foods

Prevent thermophilic spoilage.
Reduce heat treatment requirements.
Maintain product quality.
Extend shelf stability.

Alcoholic Beverages

Control bacterial contamination in beer.
Prevent spoilage in wine.
Maintain product stability.

Non-alcoholic Beverages

Preserve fruit juices.
Protect sports drinks.
Extend the shelf life of plant-based beverages.

Nisin Benefits

Technical Benefits

Antimicrobial Properties

Effective against gram-positive bacteria.
Control spore-forming organisms.
Prevent the growth of foodborne pathogens.
Active against various spoilage organisms.

Targeted Action

Specific mechanism of action.
Minimal impact on beneficial bacteria.
Natural preservation process.
Controlled antimicrobial effect.

Heat Stability

Maintain activity after pasteurization
Survive thermal processing
Stable in acidic conditions
Long-term effectiveness

Compatibility

Work synergistically with other preservatives.
Compatible with various food matrices.
Easy incorporation into products.
Stable in different formulations.

Health and Safety Benefits

Food Safety

Natural preservation method.
Reduced chemical preservative use.
Prevention of foodborne illness.
Enhanced product stability.

Health Considerations

Generally Recognized as Safe (GRAS) status.
No known allergenic properties.
Digestible protein nature.
Non-toxic characteristics.

Clean Label Benefits

Natural preservative option.
Consumer-friendly labeling.
Meets clean label demands.
Supports sustainable practices.

Nisin Used in Food

A Colostrum-Containing Cheese – Formulation by Weight

A colostrum-containing cheese, based on a weight of 100kg, includes cream cheese, 51kg; fresh cheese, 20kg; trehalose, 11kg; oligofructose, 1.1kg; sodium citrate, 0.6kg; locust bean gum, 0.18kg; carrageenan, 0.19kg; xanthan gum, 0.45kg; nisin, 0.04kg; the rest is made up with colostrum.

The raw materials of fresh cheese include: raw milk, 44kg; cream, 23kg; colostrum, 12kg; concentrated milk protein powder, 14kg; milk mineral salt, 7kg; Lactococcus lactis subsp. cremoris, 0.01kg; Lactococcus lactis subsp. diacetyl, 0.03kg; Leuconostoc mesenteroides subsp. mesenteroides, 0.02kg; rennet, 0.002kg.

The preparation process of fresh cheese is as follows:

Mixing: Mix raw milk, cream, colostrum, concentrated milk protein powder, and milk mineral salt in a mixing tank, shear at 1900rpm for 120s, then heat it to 58℃. After that, mix and stir it for 5min, and rotate at 1600rpm.
Sterilization: The liquid is sterilized at 74℃/15s.
Fermentation and curdling: Cool the liquid to 28℃, inoculate the fermentation agent (Lactococcus lactis subsp. cremoris, Lactococcus lactis subsp. diacetyl, Leuconostoc mesenteroides subsp. mesenteroides) and add rennet for fermentation. When the pH of the liquid drops to 5.1, stop the fermentation and obtain fresh cheese.

The preparation process of colostrum cheese is as follows:

Shearing: fresh cheese, cream cheese, and colostrum of fermented curd are put into a melting pot, heated to 43°C, sheared at 1900rpm for 240s, and the scraper speed is 18rpm;
Pre-dissolution: some trehalose, oligofructose, sodium citrate, and nisin raw materials are added, and dissolved at a low temperature of 43°C, the scraper speed is 20rpm, the high-speed shear speed is 2200rpm, and the shear time is 130s.
Melting: locust bean gum, carrageenan, xanthan gum, and the remaining trehalose (total colloid addition: trehalose = 1:5) are added, the material is heated to 68°C, maintained for 3min, shear speed: 1600rpm, and recirculated for 130s.
Sterilization: Sterilize the liquid material at 87°C and shear at 2100rpm for 150s.
Filling and molding: Pour the sterilized liquid material into a rod-shaped or special-shaped block mold for packaging.
Cooling: The product enters the rapid cooling tunnel to reduce the temperature to 35°C.
Packaging and storage: The product is stored in a warehouse at 2-6°C.

In conclusion, nisin’s versatility and effectiveness as a natural preservative continue to drive its adoption across various industries. Its proven safety record, combined with growing consumer demand for natural ingredients, positions it as a crucial component in modern food preservation and beyond. As a bulk nisin manufacturer and supplier in China, we sell different grades of nisin powder at bulk prices to large manufacturers and small customers worldwide.